SrasV1, Main, Exploration, bibRecord, 002913

The Route of Inoculation Determines the Tissue Tropism of Modified Vaccinia Tiantan Expressing the Spike Glycoprotein of SARS-CoV in Mice

Identifieur interne : 002913 ( Main/Exploration ); précédent : 002912; suivant : 002914

The Route of Inoculation Determines the Tissue Tropism of Modified Vaccinia Tiantan Expressing the Spike Glycoprotein of SARS-CoV in Mice

Auteurs : HUAN LIU [République populaire de Chine] ; WENBO YU [Hong Kong] ; XIAN TANG [Hong Kong] ; HAIBO WANG [Hong Kong] ; WENJIE OUYANG [République populaire de Chine] ; JINGYING ZHOU [Hong Kong] ; ZHIWEI CHEN [Hong Kong]

Source :

Journal of medical virology [ 0146-6615 ] ; 2010.

RBID : Pascal:10-0292332

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Souris, Tropisme, Glycoprotéine, Vaccin, Vaccination, Vaccine.
Wicri :
- topic : Vaccin, Vaccination.

English descriptors

KwdEn :
- Glycoprotein, Mouse, Severe acute respiratory syndrome virus, Tropism, Vaccination, Vaccine, Vaccinia.

Abstract

The live replication-competent modified vaccinia virus Tiantan (MVTT) is an attractive vaccine vector, yet little is known about its tissue tropism and pathology in vivo. Recently, we demonstrated that a recombinant MVTT expressing the spike glycoprotein of SARS-CoV (namely MVTT-S) is superior to the non-replicating modified vaccinia Ankara (MVA-S) for inducing high level of neutralizing antibodies through mucosal vaccination. In this study, we further determined the tissue tropism and safety of MVTT-S after the vaccine was administrated through various routes including: intramuscular (i.m.), intranasal (i.n.), and intravaginal (i.vag.) inoculations, respectively. Using real-time PCR, nested PCR, immunohistochemistry and in situ hybridization assays, we found that MVTT-S was able to produce a transient infection in all cases within 48 hr post-inoculation, yet the major site of viral replication in various tissues or organs was dependent on the route of viral administration. We demonstrated that i.m. injection of MVTT-S primarily targeted draining inguinal lymph nodes, whereas mucosal inoculation had broader range of tissue infections. i.n. inoculation involved infections in lungs, kidneys, spleens and cervix lymph nodes while i.vag. administration targeted uteruses, ovaries, kidneys and spleens. Critically, the infection did not cause severe pathogenic consequences in infected tissues, which was consistent to the attenuated phenotype of MVTT-S. Our findings have implications for the optimization of vaccination route and for studies on the correlation between the magnitude of immune responses and the extent of tissue involvement in vivo.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The live replication-competent modified vaccinia virus Tiantan (MVTT) is an attractive vaccine vector, yet little is known about its tissue tropism and pathology in vivo. Recently, we demonstrated that a recombinant MVTT expressing the spike glycoprotein of SARS-CoV (namely MVTT-S) is superior to the non-replicating modified vaccinia Ankara (MVA-S) for inducing high level of neutralizing antibodies through mucosal vaccination. In this study, we further determined the tissue tropism and safety of MVTT-S after the vaccine was administrated through various routes including: intramuscular (i.m.), intranasal (i.n.), and intravaginal (i.vag.) inoculations, respectively. Using real-time PCR, nested PCR, immunohistochemistry and in situ hybridization assays, we found that MVTT-S was able to produce a transient infection in all cases within 48 hr post-inoculation, yet the major site of viral replication in various tissues or organs was dependent on the route of viral administration. We demonstrated that i.m. injection of MVTT-S primarily targeted draining inguinal lymph nodes, whereas mucosal inoculation had broader range of tissue infections. i.n. inoculation involved infections in lungs, kidneys, spleens and cervix lymph nodes while i.vag. administration targeted uteruses, ovaries, kidneys and spleens. Critically, the infection did not cause severe pathogenic consequences in infected tissues, which was consistent to the attenuated phenotype of MVTT-S. Our findings have implications for the optimization of vaccination route and for studies on the correlation between the magnitude of immune responses and the extent of tissue involvement in vivo.</div>
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The Route of Inoculation Determines the Tissue Tropism of Modified Vaccinia Tiantan Expressing the Spike Glycoprotein of SARS-CoV in Mice

The Route of Inoculation Determines the Tissue Tropism of Modified Vaccinia Tiantan Expressing the Spike Glycoprotein of SARS-CoV in Mice

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri